date:Sep 14, 2012
a starting material for 2FL as part of its normal metabolism, and that suggested to us that it was possible to use E. coli to produce 2FL,explained, Yong-Su Jin a metabolic engineer also at the University of Illinois.
And we can use this technique to synthesize and study the hundreds of other HMOs in human milk too, added Miller.
Bacterial modification
The trick is to get the E. coli cells to increase their production of the starting material (GDP-fucose), which we did by overexpressing the